Escherichia coli methionine-tRNAi/methionyl tRNA synthetase pairs induced protein initiation of interest (PII) expression

Abstract The precise regulatory role in protein synthesis by facilitating interactions with mRNA codons for various tRNA modifications is unclear. We previously reported that enhanced green fluorescent (GFP) reduced GFP expression human methionine-conjugated initiator (tRNAi)/tRNA synthetase pairs under methionine-deficient conditions. Here, we investigated the effect of non-formylated Escherichia coli tRNAi on initiation interest (PII) HeLa cells intracellular L-methionine levels. found E. methionine-tRNAi counteracts methionine-tRNAi, indicating methionyl can induce due to increased stability mRNA. Both complexes could support translation without being employed introduce methionine residues subsequent elongation steps. results indicated offset and methionine-tRNAi/methionyl drive expression. Unlike were used as a positive control, mRNA, resulting cell survival. Using tRNAs functions causes heterologous origin, such from prokaryotes, modified, enhance or suppress specific proteins eukaryotic organisms into potential may possess more prominent advantage approaches control PII. This study provides new insights methionine- tRNAi/methionyl pair induced PII relative viability pave way regulate ecological/biological systems.